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. 2016 Jun 3;11(6):e0156363. doi: 10.1371/journal.pone.0156363

Fig 4. In vitro antigen recall experiments.

Fig 4

Spleen homogenates from mice immunized via the nasal route with promastigote lysate (PL) plus either wild-type CNF1 (PL + WT CNF1) or catalytically inactive CNF1 (PL + mCNF1) and infected with 108 stationary phase L. infantum metacyclic parasites were challenged with 50 μg/ml PL for 48 hours. The supernatants were collected and assayed for IL-2 (A), IFN-γ (B) and IL-4 (C) by ELISA. The bars represent the mean cytokine production ± SEM. *: p<0.05, **: p<0,01, ***: p<0,001. n = 7.