Table 1. Effects of miR-27a/b on secretion of proinflammatory cytokines in ox-LDL- treated THP-1 macrophages.
| IL-1β (ng/L) | IL-6 (ng/mL) | MCP-1 (ng/L) | TNF-a (ng/L) | |
|---|---|---|---|---|
| control | 183.04±25.07 | 0.87±0.17 | 197.37±23.89 | 91.36±10.94 |
| Mimic-neg | 181.97±19.26 | 0.86±0.13 | 199.09±20.53 | 87.17±8.03 |
| miR-27a mimic | 78.66±16.14* | 0.27±0.09* | 102.56±12.40* | 37.59±12.36* |
| miR-27b mimic | 85.28±11.30* | 0.35±0.08* | 109.28±16.86* | 40.86±8.33* |
| Inhibitor-neg | 180.26±24.82 | 0.82±0.13 | 194.89±24.20 | 88.91±8.96 |
| miR-27a inhibitor | 230.25±26.17* | 1.42±0.16* | 241.70±37.79* | 128.15±16.58* |
| miR-27b inhibitor | 228.91±25.73* | 1.38±0.10* | 232.03±38.21* | 124.57±17.94* |
| miR-27a inhibitor+LPL-siRNA | 163.73±16.25# | 0.85±0.18# | 187.52±28.83# | 78.79±11.84# |
| miR-27b inhibitor+LPL-siRNA | 159.86±12.13# | 0.80±0.14# | 185.46±28.64# | 77.57±13.04# |
| LPL | 235.49±15.59* | 1.76±0.15* | 250.95±22.15* | 140.75±21.22* |
| LPL-siRNA | 73.79±12.93* | 0.45±0.13* | 104.84±19.66* | 49.67±12.77* |
The concentrations of inflammatory cytokines secreted in 24 h culture supernatants by stimulating THP-1 macrophages with various factors were measured by ELISA. All the results are expressed as mean±S.D. from three independent experiments.
*P<0.05 vs. control,
#P<0.05 vs. miR-27a/b inhibitor.