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. 2016 Jun 3;11(6):e0154787. doi: 10.1371/journal.pone.0154787

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© 2016 Xu et al

This is an open access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.

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Fig 4 — (A) Three-week-old AtRAN gene double mutants and WT plants were cold stressed at -14°C after 4-day 4°C acclimation and then transferred back to the normal condition for recovery. Photographs of representative seedlings of the WT and transgenic lines were taken after 14 d of recovery. (B) Survival rate of the mutants and transgenic plants after freezing stress. (C) Ion leakage assay of the mutants and transgenic plants after freezing stress. The error bars show SD, and were from three independent replications. And the results were repeated three times. Asterisk (*) indicates significant difference (P < 0.05).