Table 1. Efficiency of gene targeting in goat somatic cells using TALENs.
| Cell lines (sex) | No.of G418R colonies | No.of 3’Junction PCR+ colonies (%) | No.of 5’Junction PCR+ colonies (%) | No.of LR-PCR+colonies (%) | No.of bi-allelic targeted colonies (%) | Senesceda | Targeted coloniessuitable for NT |
|---|---|---|---|---|---|---|---|
| GEF1(F) | 194 | 25(12.9) | 21(10.8) | 19(9.79) | 2(1.03) | 3 | 16 |
| GEF9(F) | 200 | 23(11.5) | 21(10.5) | 21(10.5) | 2(1.0) | 5 | 16 |
| GFF3(F) | 158 | 29(18.4) | 27(17.1) | 26(16.5) | 3(1.89) | 8 | 18 |
| GFF8(F) | 189 | 23(12.2) | 19(10.05) | 17(9.0) | 1(0.53) | 5 | 12 |
| Total | 741 | 100(13.5) | 88(11.9) | 83(11.2) | 8(1.1) | 21 | 62 |
aColonies were scored as senesced when cell numbers were not observed to increase seven days after seeding.