Table 2. Estimation of cell density (cell/mm2) in shoot base and embryogenic and non-embryogenic calli in B. rotunda.
| Tissue | Estimate cell number | Width of observation (μm) | Length of observation (μm) | Area (mm2) | Cell density (cell/mm2) |
|---|---|---|---|---|---|
| SB | 87 ± 7 | 104.8 ± 3.8 | 86 ± 3 | 0.00905 ± 0.00063 | 9680 ± 1454 |
| EC | 152.7 ± 9.5 | 107.4 ± 1.9 | 83.0 ± 1.5 | 0.00891 ± 0.00012 | 17126 ± 909 |
| DC | 170 ± 7 | 107.4 ± 2.2 | 85.8 ± 3.9 | 0.00921 ± 0.00027 | 18312 ± 695 |
| WC | 44.7 ± 4.9 | 106.5 ± 2.9 | 85.91 ± 0.86 | 0.00915 ± 0.00032 | 4896 ± 696 |
SB: shoot base; EC: embryogenic callus; DC: dry callus; WC: watery callus; ± indicates the standard deviation where n = 3 biological replicates