Skip to main content
. 2016 Jun 3;11(6):e0156072. doi: 10.1371/journal.pone.0156072

Fig 1. Generation of a Slamf1-/-Slamf5-/-Slamf6-/- triple knockout mouse line on a pure C57BL6 background by microinjecting S. pyogenes Cas9 mRNA and sgRNA into fertilized eggs.

Fig 1

(A) The Slamf1, Slamf5, Slamf6 locus is located on mouse chromosome 1. Vertical lines represent guide sequence target sites. Genomic DNA sequences of the targeted loci are shown for both a WT control and the TKO mouse. Guide sequences are in upper case, PAM sequences are bolded, predicted Cas9 cut sites are marked by arrows, and deletions are denoted by dashes. (B) Fluorescent PCR chromatograms showing sizes of target loci PCR amplicons for Slamf5 and Slamf6 in a WT control and the TKO founder. Each peak represents a differently sized mutant allele amplicon. (C) Representative CD84, Ly108, and SLAM expression on peripheral blood B cells (B220+) from TKO mice and littermates. Median fluorescence intensities of CD84, Ly108, and SLAM, n = 3–6 mice/genotype, error bars show s.e.m.