Table 1. Biochemical properties of CbMan5A in comparison with other mannanases.
| Enzyme | Organism | Optimal pH | Optimal temperature(°C) | Specific activity (unit, substrate) | kcat (s-1) | Km (mg ml-1) | Reference |
|---|---|---|---|---|---|---|---|
| CbMan5A | C. bescii | 6.5 | 90 | 1,691 (U/mg, LBG) | 1043 | 1.7 | This.work |
| 690 (U/mg, KGM) | 514 | 0.9 | This.work | ||||
| 505 (U/mg, GG) | – | – | This.work | ||||
| 1.4 (mU/mg, Avicel) | – | – | This.work | ||||
| 0.5 (mU/mg, Filter paper) | – | – | This.work | ||||
| 2.2 (mU/mg, CMC-Na) | – | – | This.work | ||||
| 5.9 (mU/mg, Beechwood xylan) | – | – | This.work | ||||
| Man5A1 | Talaromyces leycettanus | 4.5 | 90 | 2,160 (U/mg, LBG) | – | 1.9 | [19] |
| Man5A2 | Talaromyces leycettanus | 4.0 | 85–90 | 1,800 (U/mg, LBG) | – | 2.2 | [19] |
| Man5XZ3 | Aspergillus nidulans | 5.0 | 80 | 186 (U/mg, LBG) | – | 0.9 | [20] |
| ManBK | Aspergillus niger | 4.5 | 80 | – | 292 | 2.2 | [21] |
| MAN-P | Aspergillus niger | 4.5 | 80 | 3,049 (U/ml, LBG) | – | – | [22] |
| rMan5P1 | Neosartorya fischeri | 4.0 | 80 | 1,703 (U/mg, LBG) | – | 0.8 | [23] |
| Man5XZ7 | Thielavia arenaria | 5.0 | 75 | – | – | 5.3 | [24] |
| Man5A | Humicola insolens | 5.5 | 70 | 1,122 (U/mg, LBG) | – | 1.5 | [25] |
| AaManA | Alicyclobacillus acidocaldarius | 5.5 | 65 | – | 340 | 2.4 | [26] |
| ManA | Bacillus subtilis | 7.0 | 60 | 415.2 (U/ml, LBG) | – | – | [27] |
| MANN | Aspergillus sulphureus | 2.4 | 50 | 366 (U/mg, LBG) | – | 0.9 | [28] |
| ManA | Xanthomonas campestris | 7.0 | 37 | 282.55 (U/mg, LBG) | – | 0.88 | [29] |