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. 2016 Jun 3;11(6):e0156935. doi: 10.1371/journal.pone.0156935

Fig 1. Bone marrow cells express smooth muscle cell markers upon short-term culture.

Fig 1

qRT-PCR analysis of SMC marker expression in cultured bone marrow cells and murine aortic smooth muscle cells (SMCs) after the indicated culture period. Expression of α-SMA and SM22-α (A) and SM-MHC (B) was normalized relative to GAPDH (* p < 0.05, ** p < 0.01 compared to 0 d, Dunnett’s test, n = 3). The number of α-SMA+ cells was quantified for the indicated time (C). Western blot detection of α-SMA (D) and SM22-α (E) expression of mouse bone marrow cells cultured for the indicated period.