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Top: Viral titers expressed as median (50%) egg-infectious dose equivalent by qRT-PCR (see Materials and Methods) [27, 28]. Bottom: viral titers re-isolated in MDCK cells. Horizontal bars represent mean antibody titer for the group (n = 7). dpc, days post challenge. TCID50, median (50%) tissue culture infective dose. *p<0.05. **p<0.001.
