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. 2016 Jun 3;11(6):e0156560. doi: 10.1371/journal.pone.0156560

Fig 4. Activation of Cry1Ac protoxin by midgut extract from the susceptible strain LF of H. armigera with and without the chymotrypsin inhibitor TPCK.

Fig 4

(A) Representative SDS-PAGE gel. (B) Percentage activation (mean and SE) based on optical density of the activated toxin band (65 kDa) relative to the band with only protoxin (lane 2) calculated by Image J quantification from three replicates. For each incubation period (30 min for lanes 3–5 and 2 h for lanes 6–8), the inhibitor TPCK had no significant effect on activation (t-tests, P > 0.05). Lane 1, molecular weight markers (kDa); Lane 2, Cry1Ac protoxin; Lanes 3 and 6, Cry1Ac protoxin and midgut extract; Lanes 4 and 7, Cry1Ac protoxin and 10:1 midgut extract + TPCK; Lanes 5 and 8, Cry1Ac protoxin and 1:1 midgut extract + TPCK.