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. 2016 Jun 3;11(6):e0156560. doi: 10.1371/journal.pone.0156560

Fig 6. Time course of the activation of Cry1Ac protoxin by midgut extracts from the susceptible LF strain and resistant LF120 strain of H. armigera.

Fig 6

(A) Representative SDS-PAGE gel. (B) Percentage activation (mean and SE) based on optical density of the activated toxin band (65 kDa) relative to the band with only protoxin (P) calculated by Image J quantification from three replicates. M: Protein marker; P: Only protoxin; 30, 60, 120 and 180 were the incubation time points (min). The asterisk indicates significantly lower activation for LF120 than LF at 30 min. Activation did not differ significantly between strains at 60, 120, and 180 min.