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. 2016 Jun 3;11(6):e0157016. doi: 10.1371/journal.pone.0157016

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© 2016 Haycocks, Grainger

This is an open access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.

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Fig 3 — DNAse I footprint analysis of the PaatS region. The lane labelled ‘G+A’ is a Maxim/Gilbert G+A sequencing reaction. Lane 1 shows the cleavage pattern obtained from aatS1 DNA digested with DNAse I in the absence of CRP. Lanes 2–6 show DNAse I cleavage patterns generated in the presence of increasing concentrations of CRP (0.35 μM, 0.7 μM, 1.05 μM, 1.4 μM and 2.1 μM). The predicted CRP site is indicated by a hashed grey bar. DNA protection is indicated by black dashes, hypersensitive bends are highlighted by stars.