Table 2. Intraclass Correlation Coefficient (ICC) for one sample from each tumour (ICCsingle) and for three different samples from each tumour (ICCmean) as obtained by RT-qPCR and ISH.
| Method | ICC (single) | ICC (mean) | |
|---|---|---|---|
| miRNA-21 | PCR | 0.650 | 0.848 |
| ISH | 0.632 | 0.838 | |
| miRNA-125b | PCR | 0.453 | 0.713 |
| ISH | 0.281 | 0.540 | |
| miRNA-145 | PCR | 0.134 | 0.317 |
| ISH | 0.264 | 0.518 | |
| miRNA-630 | PCR | 0.284 | 0.543 |
| ISH | 0.222 | 0.461 | |
| miRNA-31 | PCR | 0.782 | 0.915 |
The ICCsingle was higher than 50% for miRNA-21 (RT-qPCR: 65%, ISH: 63%) and miRNA-31 (RT-qPCR: 78%), and for miRNA-125b, miRNA-145, and miRNA-630, it was lower than 50%.
As to ICCmean it was higher than 50% for miRNA-21(RT-qPCR and ISH), miRNA-125b (RT-qPCR and ISH), miRNA-145 (ISH), miRNA-630 (RT-qPCR), and miRNA-31 (RT-qPCR) and lower than 50% for miRNA-145 (RT-qPCR) and miRNA-630 (ISH). As shown in Table 2, the ICCmean is generally higher than the ICCsingle.