Fig. 7.

Inhibition of endocytosis leads to intestinal dysplasia. (A) Expression of dominant-negative shibire (dynamin) leads to a striking upregulation of mitotic activity and large-scale accumulation of small diploid esg-GFP-positive stem cells in 7-day-old animals. Boxed areas are shown enlarged in the lower panels. (B) Knockdown of Rab7 in esg-GFP-positive cells leads to intestinal dysplasia in 3-week-old animals that is indistinguishable from that seen in UVRAG RNAi animals of a similar age. Note that the analysis of ISCs and EBs in Rab7 loss-of-function posterior midguts is compared to the same control animals as shown in Fig. 1A as experiments were performed in parallel.