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. 2016 May 1;9(5):541–552. doi: 10.1242/dmm.024513

Fig. 4.

Fig. 4.

Immune cell infiltration is reduced by HDAC inhibitors in abdominal aorta from Ang-II-infused ApoE−/− mice. ApoE−/− mice were infused with saline or Ang II. Ang-II-infused mice were treated or not with MC-1568 (MC) or MS-275 (MS). (A) Representative images corresponding to macrophage (Mac-3, upper panels), lymphocyte (CD3, middle panels) and neutrophil [elastase, neutrophil expressed (Elane); lower panels] infiltration analysed by immunohistochemistry. Arrows indicate positive cells for each marker. Scale bars: 50 μm. (B-D) Histograms show the quantification of cells positive for Mac-3 (B), CD3 (C) and neutrophil elastase (D) per aortic section (n=5). (E,F) The aortic expression of EMR-1, a macrophage marker (E), and Elane, a neutrophil marker (F), were evaluated by real-time PCR and normalised to GAPDH expression (n=8). Values shown are mean±s.e.m. *P<0.05 vs saline; #P<0.05 vs Ang II (t-test and one-way ANOVA).