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. 2016 Apr 10;67(11):3277–3288. doi: 10.1093/jxb/erw142

Fig. 1.

Fig. 1.

Confirmation of ubc22 T-DNA insertion mutants. (A) Schematic representation of the genomic structure of UBC22 with the locations of primers and insertion sites of T-DNA in the ubc22-1 and ubc22-2 mutants shown. Closed boxes, exons; open boxes, introns; shadowed boxes, untranslated regions; HW1089 and HW1090, gene-specific primers for UBC22; SW17, T-DNA left border primer for SALK_011800 line; HW659, T-DNA left border primer for GK_642C08 line. (B) Characterization of ubc22 T-DNA lines by genomic PCR. Plant lines used are indicated above the panels, and the primers to the right of the panels. M, DNA size marker. (C) Characterization of ubc22 T-DNA lines by RT-PCR; 30 PCR cycles were used. PCR amplification of a reference At4g33380 cDNA is shown in the upper panel and amplification of UBC22 cDNA is shown in the lower panel.