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. 2016 Apr 10;67(11):3277–3288. doi: 10.1093/jxb/erw142

Fig. 3.

Fig. 3.

Complementation of the ubc22-1 mutant by Pro UBC22 ::HA-UBC22. For complementation, Pro UBC22 ::HA-UBC22 was introduced into the ubc22-1 mutant. T2 plants of independent lines were used for further analysis. (A) Silique length of WT, ubc22-1, and three individual complementation lines (#7, #10 and #21). Approximately six siliques from each plant were measured, from at least four different plants in each line. Student’s t-test was performed to determine whether there was a significant difference in silique length between the complementation lines and the mutant. ** Significant difference at P<0.01. (B) Representative siliques from WT, ubc22-1, and three complementation lines. Scale bar, 1mm. (C) RT-PCR analysis of WT, ubc22-1, and three independent complementation lines. PCR amplification of a reference At4g33380 cDNA is shown in the upper panel and amplification of UBC22 cDNA is shown in the lower panel. Plant lines used are indicated above the panels. M, DNA size marker.