Table 2.
Analysis of seed development in siliques from reciprocal crosses and self-fertilized siliques of heterozygous plants
| Female × male | Seeds/ovules counted | Normal seeds | Aborted ovules | Aborted ovules per silique | Aborted seeds | Silique length (mm) |
|---|---|---|---|---|---|---|
| WT × WT | 1265 (100%) |
1264 (99.92%) |
1 (0.08%) |
0.1±0.2 | 0 | 16.2±0.2 |
| ubc22-1 × WT | 1165 (100%) |
89 (7.64%) |
1057 (90.73%) |
52.9±2.9** | 19 (1.63%) |
6.4±1.0 |
| WT × ubc22-1 | 1228 (100%) |
1154 (93.97%) |
71 (5.78%) |
3.6±2.5** | 3 (0.24%) |
15.7±0.6 |
|
ubc22-1/+ × ubc22-1/+
(self-fertilized) |
1196 (100%) |
1038 (86.79%) |
157 (13.13%) |
7.9±2.9** | 1 (0.08%) |
14.9±0.6 |
Reciprocal crosses were made between WT and homozygous ubc22-1 mutant plants. Heterozygous plants (ubc22-1/+) were also included in the analysis as a comparison. Twenty fully elongated siliques from each type were used for determining the number of normal seeds, aborted ovules, and aborted seeds, as well as silique length. Student’s t-test was performed on the number of aborted ovules per silique from reciprocal crosses and heterozygous plants compared with WT plants. ** Significant difference at P<0.01.