Table 3.
Female gametophyte development in WT and ubc22-1 mutant plants
| Line | Total number | Female gametophyte stage | No nucleus |
One nucleus |
Two nuclei |
||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| FG1 | FG2 | FG3 | FG4 | FG5 | FG6 | FG7 | |||||
| WT (%) |
270 (100) |
0 | 0 | 0 | 0 | 1 (0.4) |
0 | 268 (99.3) |
1 (0.4) |
0 | 0 |
|
ubc22-1
(%) |
331 (100) |
2 (0.6) |
2 (0.6) |
4 (1.2) |
8 (2.4) |
23 (7.0) |
3 (0.9) |
19 (5.7) |
211 (63.8) |
47 (14.2) |
12 (3.6) |
Embryo sacs from flowers just before opening were dissected, prepared, and then observed under a microscope equipped with DIC optics. The stages of embryo sacs were determined based on the number of cells and their configurations according to the description of Christensen et al. (1997).