Table 4.
Megaspore mother cell (MMC) development in WT and ubc22-1 mutant ovules
| Line | Total number | No nucleus | One nucleus | Two nuclei |
|---|---|---|---|---|
| WT | 267 (100%) |
40 (15.0%) |
211 (79.0%) |
16 (6.0%) |
| ubc22-1 | 138 (100%) |
20 (14.5%) |
112 (81.2%) |
6 (4.3%) |
Flowering buds were first checked for ovule developmental stage, and the sixth bud from the newly opened flower was used. The buds were dissected, prepared, and then observed under a microscope equipped with DIC optics. The number of ovules in different categories, in terms of the presence and appearance of MMC, was determined.