Fig. 3.
The expression levels and protein contents of APX were analysed in transgenic ovules and fibres. (A) Detection of GhAPX1A T /D T expression level by northern blotting. The ORF sequence of GhAPX1A T was used as the probe. (B) Detection of GhAPX1AT/DT proteins by western blotting. Anti-histone3 (His3) (Abcam, San Francisco, CA, USA) was used as the reference. (C) RT-PCR analysis of cytosolic APX transcript levels during different developmental stages. OA15/16/17/18, individual over-expression lines; IAU20/21/22, individual GhAPX1A T /D T RNAi lines; IAO24/167, individual cytosolic APXs RNAi lines; Null, transgenic negative control; WT, wild type cotton YZ1.