Fig. 5.

Sensitivity of transgenic cotton to oxidative stress and RT-qPCR validation of differentially expressed genes (DEGs). (A) Fibres in the OA line were less sensitive to oxidative stress in the presence of 1mM H₂O₂ in ovule culture medium. IG: 5 µM IAA and 0.5 µM GA₃; IGH: 5 µM IAA, 0.5 µM GA₃ and 1mM H₂O₂. Scale bar: 5mm. (B) The fibre length analysis was performed after culturing the ovules with 1mM H₂O₂. RL indicates the fibre length percentage in response to treatments compared with non-treatment for each line (mean±sd, n=8).) Values with different letters (a, b, c and d) above the bars in the histogram indicate significant differences (Duncan’s multiple comparisons, P<0.05). (C-E) RT-qPCR verification of DEGs related to signal transduction (C), stress response (D) and secondary cell wall synthesis genes (E) in 10 DPA fibres.