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. 2016 Apr 15;67(11):3303–3312. doi: 10.1093/jxb/erw148

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© The Author 2016. Published by Oxford University Press on behalf of the Society for Experimental Biology.

This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/3.0/), which permits unrestricted reuse, distribution, and reproduction in any medium, provided the original work is properly cited.

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Fig. 3. — (a) SDS-PAGE of the two SEC fractions straddling the elution peaks (P₁ and P₂). The SEC separation leads to improved purity as can be seen by the presence of a single band with an apparent mass of ~90kDa. Two different molecular markers, indicated by M₁ and M₂, were used as a reference. (b) Glycoprotein staining assay on SDS-PAGE of the SEC fractions. The marker (M₂), being composed of non- glycosylated proteins, is used as a negative control.