Table 1.
Diatom strains, location of isolation, and Rubisco kinetics measured at 25 °C
| k cat c | Kc | k cat o | K O | SC/O | K C 21%O2 | k cat c/K C | k cat c/K C 21%O2 | ||
|---|---|---|---|---|---|---|---|---|---|
| Species | Location isolated | (s−1) | (μM) | (s−1) | (μM) | (mol mol−1) | (μM) | (mM s−1) | (mM s−1) |
| Diatoms (Bacillariophyta) | |||||||||
| Thalassiosira weissflogii (CCMP 1336)a | Gardiners Island, Long Island, NY, USA | 3.2±0.2 | 65±3 | 1.3 | 2032±458 | 79±1 | 73 | 49 | 44 |
| Thalassiosira oceania (CS-427)a | Little Swan port, Tasmania, Australia | 2.4±0.4 | 65±5 | 0.4 | 954±228 | 80±2 | 83 | 36 | 28 |
| Skeletonema marinoi (CCMP 1332)a | Milford, CT, USA | 3.2±1.1 | 68±8 | ND | 883±346 | ND | 88 | 47 | 36 |
| Chaetoceros calcitrans (CCMP 1315) | Collection site unknown | 2.6±0.2 | 25±1 | 0.8 | 413±53 | 57±11 | 41 | 103 | 64 |
| Chaetoceros muelleri (CCMP 1316) | Oceanic Institute, HI, USA | 2.4±0.3 | 23±1.5 | 0.5 | 425±67 | 96±2 | 37 | 104 | 65 |
| Chaetoceros calcitrans (CS-178) | Unknown (but should be synonymous with CCMP 1315) | 3.4±0.6 | 31±2 | 0.7 | 490±54 | 75±1 | 47 | 112 | 74 |
| Bellerochea cf. horologicalis (CS-874/01) | Ilbilbie, Queensland, Australia | 2.1±0.2 | 50±4 | ND | 764±190 | ND | 67 | 41 | 31 |
| Phaeodactylum tricornutum (UTEX 642) | Plymouth, UK | 3.2±0.9 | 36±1 | 0.5 | 592±44 | 108±2 | 52 | 88 | 62 |
| Phaeodactylum tricornutum (CS-29) | Unknown, UK | 3.3±0.6 | 41±1 | 0.5 | 664±54 | 116±2 | 57 | 81 | 59 |
| Fragilariopsis cylindrus (CCMP 1102) | Islas Orcadas Cruise, Station 12 | 3.5±0.3 | 64±8 | 0.5 | 667±255 | 77±1 | 88 | 55 | 40 |
| Cylindrotheca fusiformis (CS-13) | Halifax, Canada | 3.7±0.2 | ND | ND | ND | 79±1 | ND | ND | ND |
| Higher plants (controls) | |||||||||
| Nicotiana tabacum (tobacco, C3) | 3.1±0.3 | 9.7±0.1 | 1.1 | 283±15 | 82±1 | 18.3 | 316 | 167 | |
| Zea mays (maize, C4) | 5.5±0.2 | 19.0±0.6 | 1.4 | 397±59 | 88±2 | 31 | 289 | 177 |
a Belonging to the order Thalassiosirales;
Values shown are average of measurements from n ≥3 (± SD) biological repeat samples (see figure legends); ND, not determined
KC 21%O2 was calculated as K C(1+O2/K O) assuming air-saturated O2 levels in H2O of 252 µM