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. 2016 May 2;67(11):3497–3508. doi: 10.1093/jxb/erw172

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© The Author 2016. Published by Oxford University Press on behalf of the Society for Experimental Biology.

This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/3.0/), which permits unrestricted reuse, distribution, and reproduction in any medium, provided the original work is properly cited.

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Fig. 1. — JA and JA-Ile increase in embryos of imbibed wheat grains in response to cold. (A) The effect of stratification on the germination of dormant grain. The GI was calculated over 7 d imbibition at 20 ^oC after different periods of cold treatment (0–84h). (B) JA content in embryos isolated from Sunstate wheat grains imbibed for 96h continuously in the cold or at 20 ^oC. (C) Changes in JA content in embryos isolated from wheat grains that have been stratified for different times (12, 24, 48, and 72h) prior to imbibition at 20 ^oC. (D) Changes in JA and JA-Ile content in embryos of wheat grains in response to 48h stratification. Grains were imbibed at 4 ^oC for 48h and then transferred to room temperature (RT) or kept at 4 ^oC. Values are means ±SE (n=4), and each replicate was obtained from 20 grains (A) and 40 embryos (B–D).