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. 2016 Apr 28;67(11):3471–3479. doi: 10.1093/jxb/erw173

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© The Author 2016. Published by Oxford University Press on behalf of the Society for Experimental Biology.

This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/3.0/), which permits unrestricted reuse, distribution, and reproduction in any medium, provided the original work is properly cited.

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Fig. 2. — NtMPK4L activity is upregulated by wounding. Phosphorylation of MBP as a substrate was detected by autoradiography. (A) Tobacco leaves were wounded using a cork borer. After 0 and 10min, the leaf discs were used for the measurement of NtMPK4 or NtMPK4L activity. (B) Tobacco leaves were wounded using a cork borer. After 0, 5, 10, 30, 60 and 120min, the leaf discs were used for the measurement of NtMPK4L activity. (C) In vitro activation of His-NtMPK4 or His-NtMPK4L by GST-SIPKK (WT) or GST-SIPKK^EE (EE) was examined. (D) Tobacco leaves expressing HA-SlMPK6 or HA-SlMPK6^Y203F by agroinfiltration were wounded using a cork borer. After 0, 10 and 30min, immune complexes prepared from the leaf discs were used for the measurement of SlMPK6 or SlMPK6^Y203F activity. Tobacco leaves expressing HA-sGFP (sGFP) by agroinfiltration were used as negative control.