Table 1.
Gene | Sequences (5′-3′) | Primer length (bp) | Product length (bp)* |
---|---|---|---|
miR-20a | RT: GTCGIATCCAGTGCAGGGTCCGAGGTATTCGCACTGGATACGACTACCTG | 50 | 62 |
For: GCGGCGGTAAAGTGCTTATAGTG | 23 | ||
Rev: TGCAGGGTCCGAGGTAT | 18 | ||
U6 | For: CTCGCTTCGGCAGCACA | 17 | 94 |
Rev: AACGCTTCACGAATTTGCGT | 20 |
Note:
Product length data is not given for some sequences as the “Product length” column refers to digested product length.
Abbreviations: bp, base pair; For, forward; miR, microRNA; Rev, reverse; RT, reverse transcription.