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. 2016 May 27;9:3067–3076. doi: 10.2147/OTT.S96861

Table 1.

Primer sequences and amplification product length

Gene Sequences (5′-3′) Primer length (bp) Product length (bp)*
miR-20a RT: GTCGIATCCAGTGCAGGGTCCGAGGTATTCGCACTGGATACGACTACCTG 50 62
For: GCGGCGGTAAAGTGCTTATAGTG 23
Rev: TGCAGGGTCCGAGGTAT 18
U6 For: CTCGCTTCGGCAGCACA 17 94
Rev: AACGCTTCACGAATTTGCGT 20

Note:

*

Product length data is not given for some sequences as the “Product length” column refers to digested product length.

Abbreviations: bp, base pair; For, forward; miR, microRNA; Rev, reverse; RT, reverse transcription.