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. 2016 Jan 29;48(1):e206. doi: 10.1038/emm.2015.102

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Copyright © 2016 KSBMB.

This work is licensed under a Creative Commons Attribution-NonCommercial-NoDerivs 4.0 International License. The images or other third party material in this article are included in the article's Creative Commons license, unless indicated otherwise in the credit line; if the material is not included under the Creative Commons license, users will need to obtain permission from the license holder to reproduce the material. To view a copy of this license, visit http://creativecommons.org/licenses/by-nc-nd/4.0/

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NF-κB-dependent genes induced by TCR stimulation are enhanced in CHMP5-knockdown Jurkat cells. (a–f) RNA was extracted from Ctrl and CHMP5^KD Jurkat cells stimulated with anti-CD3/anti-CD28 for 1, 3, 9, 12 and 24 h, followed by quantitative RT-PCR analysis of the effects of CHMP5 knockdown (a, CD69; b, HLA-B; c, NFKB2; d, NFKB1; e, LTA; f, RELB). Data represent the average of data from two independent experiments, each conducted in triplicate. Error bars represent the mean±s.d. of six samples. *P<0.01 and **P<0.05.