Table 1. Sequences and accession numbers for the primers (forward, FOR; reverse, REV) used in real-time RT–PCR.
| Gene | Primer sequences | Accession no. |
|---|---|---|
| SIRT1 | FOR:GACACAGAGACGGCTGGAA REV: CAGACCCTCAAGCCATGTTT | NM_ 019812 |
| TNF-α | FOR: GCTGTCCCTGCGCTTCA REV:CTCGTCCCCAATGACATCCT | NM_ 013693 |
| IL-1β | FOR:CCATGGCACATTCTGTTCAAA REV:GCCCATGAGAGGCAAGGA | NM_ 008361 |
| IL-2 | FOR:AACTCCCCAGGATGCTCAC REV:CGCAGAGGTCCAAGTTCATC | NM_008366 |
| IL-4 | FOR:TCGGCATTTTGAACGAGGTC REV:GAAAAGCCCGAAAGAGTCTC | NM_021283 |
| IL-6 | FOR: CCACGGCCTTCCCTACTTC REV: TTGGGAGTGGTATCCTCTGTGA | NM_ 031168 |
| IL-13 | FOR: CGCAAGGCCCCCACTAC REV:AAAGTGGGCTACTTCGATTTTGG | NM_008355 |
| IL-17 | FOR: TGAGCTTCCCAGATCACAGA REV: TCCAGAAGGCCCTCAGACTA | NM_ 010552 |
| IL-23 | FOR: GCTCCCCTTTGAAGATGTCA REV: GACCCACAAGGACTCAAGGA | NM_ 031252 |
| IL-10 | FOR: TGTCAAATTCATTCATGGCCT REV: ATCGATTTCTCCCCTGTGAA | NM _010548 |
| IFN-γ | FOR: TGAGCTCATTGAATGCTTGG REV: ACAGCAAGGCGAAAAAGGAT | NM_ 008337 |
| MCP-1 | FOR: TGTCACCCTTGGAGCTCATG REV: TTTTTCGACTTTTATCCTCTGTTG | NM_ 011333 |
| MMP-3 | FOR: CCCCTGATGTCCTCGTGGTA REV: GCACATTGGTGATGTCTCAGGTT | NM_ 010809 |
| MMP-13 | FOR: GGTCCTTGGAGTGATCCAGA REV: TGATGAAACCTGGACAAGCA | NM_ 008607 |
| T-bet | FOR: TTCCCATTCCTGTCCTTCAC REV: CCACATCCACAAACATCCTG | NM_ 019507 |
| ROR-γT | FOR: TGAGGCCATTCAGTATGTGG REV: CTTCCATTGCTCCTGCTTTC | NM_011281 |
| GATA3 | FOR: GGAAACTCCGTCAGGGCTA REV: AGAGATCCGTGCAGCAGAG | NM_008091 |
| Foxp3 | FOR: CCTGCCTTGGTACATTCGTG REV: TGTTGTGGGTGAGTGCTTTG | NM_054039 |
| GAPDH | FOR: CGTCCCGTAGACAAAATGGT REV: TTGATGGCAACAATCTCCAC | NM_008084 |
Abbreviations: GAPDH, glyceraldehyde-3-phosphate dehydrogenase; MCP-1, monocyte chemoattractant protein-1.