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. 2016 Mar 25;48(3):e222. doi: 10.1038/emm.2015.126

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Copyright © 2016 KSBMB.

This work is licensed under a Creative Commons Attribution-NonCommercial-NoDerivs 4.0 International License. The images or other third party material in this article are included in the article's Creative Commons license, unless indicated otherwise in the credit line; if the material is not included under the Creative Commons license, users will need to obtain permission from the license holder to reproduce the material. To view a copy of this license, visit http://creativecommons.org/licenses/by-nc-nd/4.0/

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miR-19a and miR-96 enhance proliferation and tumorigenicity of hepatocellular carcinoma (HCC) in vitro. (a) HepG2 and Hep3B liver cancer cells were transfected with miR-19a mimics, miR-96 mimics, miR-19a inhibitors and miR-96 inhibitors. Cell viability (3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT)) assays were performed 1, 2, 3 and 7 days after transfection. (b) Soft agar colony formation assays were conducted after HepG2 cells were transfected with miR-19a mimics, miR-96 mimics, miR-19a inhibitors and miR-96 inhibitors. Three independent experiments were performed. The mean±s.d. is shown, and data were analyzed using Student's t-test.