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. Author manuscript; available in PMC: 2016 Jun 3.
Published in final edited form as: Science. 2016 Jan 15;351(6270):271–275. doi: 10.1126/science.aad4076

Fig. 1. Muscle-specific expression of the DWORF peptide.

Fig. 1

(A) Northern blot of adult mouse tissues showing Dworf RNA expression. (B) Western blot of adult mouse tissues with the DWORF-specific antibody reveals a single band at the predicted size of 3.8 kD. Quad, quadriceps; G/P, gastrocnemius/plantaris;TA, tibialis anterior; EDL, extensor digitorum longus. (C) Detection of Dworf RNA by qRT-PCR in 6-month-old WT and αMHC-CnA mice. Mean ± SEM; WT, n = 4; Tg, n = 5. (D) Western blot analysis of heart homogenates from WT and αMHC-calcineurin mice immunoblotted with DWORF-specific antibody. (E) qRT-PCR analysis of human ischemic heart failure tissue showing reduced DWORF mRNA in failing hearts, whereas atrial natriuretic peptide (NPPA) is significantly increased. Means ± SEM; nonfailing, n = 8; failing, n = 8.