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. Author manuscript; available in PMC: 2017 May 20.
Published in final edited form as: J Genet Genomics. 2016 Mar 8;43(5):319–327. doi: 10.1016/j.jgg.2016.02.004

Fig. 3. Delivery of Cas9 protein through electroporation achieved high efficiency of targeted small DNA fragment insertion.

Fig. 3

A: Schematic of the Rosa26 target sequence and DNA oligo donor. The protospacer sequence is colored in blue and PAM sequence is in red. The LoxP site in the DNA oligonucleotide is underlined. B: A representative image shows the analysis result of the sizes of the PCR products using TIDE. The red bar at the 34 bp insertion indicates potential insertion of LoxP site. The result of one of the pups with one time of electroporation was shown (A3). C: PCR products were cloned and individual clones were sequenced. Sequencing trace from clones A3-1 is shown. The successfully inserted LoxP site is underlined.