. Author manuscript; available in PMC: 2017 May 20.

Published in final edited form as: J Genet Genomics. 2016 Mar 8;43(5):319–327. doi: 10.1016/j.jgg.2016.02.004

Table 2.

CRISPR/Cas9 mediated large segment deletion of Smc1b through electroporation of Cas9 protein in an inbred strain.

Time of electroporation	Pseudo-ID	Number of embryos transferred	Number of mice born	Live birth rate (%)	Percentage of indel^a (%) (gRNA1/gRNA2)	Percentage of 2.2 kb deletion^b (%)
1	1	15	6	33.33	80.00/60.00	0
	2	15	4
4	3	15	4	23.33	28.57/42.85	14.29
	4	15	3
6	5	15	5	33.33	70.00/70.00	30.00
	6	15	5

The percentage of mice with indel mutation at the targeting site of either gRNA1 or gRNA2.

The percentage of mice with the desired 2.2 kb deletion at the targeting site.