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. Author manuscript; available in PMC: 2017 Aug 1.
Published in final edited form as: J Comp Neurol. 2015 Dec 29;524(11):2266–2280. doi: 10.1002/cne.23945

Figure 9. SynDIG4 is enriched at extrasynaptic AMPARs.

Figure 9

A, B, D, E, G: Representative dendrite stretches of dissociated primary cortical neurons (10 DIV) stained with antibodies against SynDIG4 (SD4) or SynDIG1 (SD1) and other synaptic markers. A, B: SD4/ L102/45, D, E: SD4/NG5-73, and G: SD1. GluA1, GluA2, and PSD93 were used as post-synaptic markers, with vGlut1 as a presynaptic marker. Synapses were defined as overlapping pre- and post-synaptic markers.

C, F, H: Quantification of (A-B, D-E, and G), respectively, represented as the fraction of SynDIG1 or SynDIG4 colocalized with the indicated synaptic marker (n = 10 for each staining set). Scale bar = 10 μm.