Table 1.
Primary antibodies Used in This Study
| Target | Host | Isotype | Type (clone) | Immunogen | Source | Cat. No. | RRID | Form | IB dilution | ICC dilution | IHC dilution |
|---|---|---|---|---|---|---|---|---|---|---|---|
| SynDIG4 (L102/45) | mouse | IgG2A | mono (L102/45) |
Amino acids 1-66 rat MSSEKSGLPDSVPHTSPPPYNAPQPPAEPPIPPPQT APSSHHHHHHHYHQSGTATLPRLGAGGLAS |
Trimmer Lab (available at NeuroMab) |
73-409 | AB_2491106 | TC supe | Neat | 1:2 | 1:2 |
| SynDIG4 (NG5.1) | rabbit | poly | RGPSSSATLPRPPH | Smit Lab (GenScript) |
custom | 1:1k | 1:100 | ||||
| SynDIG4 (NG5.73) | rabbit | poly | MSSEKSGLPDSVPH | Smit Lab (GenScript) |
custom | 1:1k | 1:100 | ||||
| HA | rat | mono (3F10) |
Amino acids 76-111 of X47 hemaglutinin 1 YPYDVPDYA |
Roche | 11867431001 | AB_390919 | purified | 1:1k | 1:100 | ||
| β- tubulin | mouse | IgG1 | mono | Amino acids 412-430 bovine SNMNDLVSEYQQYQDATA |
Millipore | 05-661 | AB_309885 | protein G purified |
1:5k | ||
| SynDIG1 | mouse | IgG2A | mono (L42/17) |
Amino acids 1-183 of mouse MDGIIEQKSVLVHSKISDAGKRNGLINTRNFMAE SRDGLVSVYPAPQYQSHRLVASAAPGSLEGGRS EPVQQLLDPNTLQQSVESHYRPNIILYSDGVLRS WGDGVATDCCETTFIEDRSPTKDSLEYPDGKFID LSGDDIKIHTLSYDVEEEEELQELESDYSSDTESE DNFLMMPPRDHLG |
Trimmer Lab (available at NeuroMab) |
75-251 | AB_10999753 | Purified IgG | 1:1k | 1:100 | |
| GluA1 | rabbit | poly | human cytoplasmic domain | Millipore | AB1504 | AB_2113602 | 1:3k | 1:100 | 1:200 | ||
| GluA2 | mouse | IgG1 | mono (L21/32) |
Amino acids 834-883 rat FCYKSRAEAKRMKVAKNAQNINPSSSQNSQNFA TYKEGYNVYGIESVKI |
NeuroMab | 75-002 | AB_2232661 | Purified IgG | 1:3k | 1:100 | |
| PSD-95 | mouse | IgG2A | mono (K28/43) |
Amino acids 77-299 human PSD-95 FSIAGGTDNPHIGDDPSIFITKIIPGGAAAQDGRLR VNDSILFVNEVDVREVTHSAAVEALKEAGSIVRL YVMRRKPPAEKVMEIKLIKGPKGLGFSIAGGVG NQHIPGDNSIYVTKIIEGGAAHKDGRLQIGDKILA VNSVGLEDVMHEDAVAALKNTYDVVYLKVAK PSNAYLSDSYAPPDITTSYSQHLDNEISHSSYLGT DYPTAMTPTSPRRYSPVA |
NeuroMab | 75-028 | AB_2307331 | Purified IgG | 1:20k | ||
| Synaptophysin 1 | mouse | IgG1 | mono (7.2) | cytoplasmic tail | Synaptic Systems |
101011 | AB_887824 | Purified IgG | 1:10k | ||
| vGlut1 | guinea pig |
poly | C-terminus of rat vGlut1 | Millipore | AB5905 | AB_2301751 | serum | 1:500 | 1:500 | ||
| PSD-93 | mouse | IgG1 | mono (N18/30) |
full length rat | NeuroMab | 75-057 | AB_2277296 | Purified IgG | 1:100 | ||
| DAPI | 1:2k |