(A–E). We analyzed RNA expression of several erythroid-related genes (BCL11a (A), cMYB (B), GATA1 (C), GATA2 (D), and KLF1 (E)) after 13-day erythroid differentiation (day 30) following CD34 or GPA selection from ES sac at day 15. We observed higher BCL11a expression, higher cMYB expression, lower GATA1 expression, lower GATA2 expression, and lower KLF1 expression in the CD34+ or GPA− cell fraction, compared to the CD34− or GPA+ cell fraction.