Table 2.
Relationship among sSC neuronal markers in layer 1.
Gene (X) | % (Rorβ+ ∩ X+) / Rorβ+ | % (Rorβ+ ∩ X+) / X+ | % (Ntng2+ ∩ X+) / Ntng2+ | % (Ntng2+ ∩ X+) / X+ |
---|---|---|---|---|
Rorβ | 83.6 ± 5.3 | 81.1 ± 3.0 | 78.6 ± 4.6* | 50.3 ± 8.1# |
Ntng2 | 51.5 ± 6.4# | 81.8 ± 4.3* | 82.5 ± 3.4 | 89.3 ± 1.9 |
Cdh7 | 53.3 ± 3.5 | 70.1 ± 5.4 | 45.8 ± 3.7 | 43.5 ± 3.9 |
Cntn3 | 64.4 ± 3.0 | 75.8 ± 4.0 | 58.9 ± 9.7 | 50.3 ± 5.5 |
Cdh6 | 7.9 ± 2.3 | 14.3 ± 3.4 | 9.6 ± 2.1 | 18.0 ± 3.6 |
Pcdh20 | 1.4 ± 1.4 | 2.3 ± 2.1 | 2.3 ± 1.3 | 2.5 ± 1.6 |
SP | 2.2 ± 1.9 | 3.4 ± 3.1 | 4.9 ± 2.5 | 7.8 ± 4.3 |
Summary of results from double In situ hybridization using either Rorβ (2nd and 3rd columns) or Ntng2 (4th and 5th columns) as the second probe. The number in each case indicates the average overlap (∩) between the second probe and each first probe (X) or vice versa. Data (mean ± SD) were averaged across 7–12 sections from at least 3 independent experiments. Symbols (*, #) indicate cases where two different second probes produced similar degrees of overlapping expression between Rorβ and Ntng2, suggesting that our double In situ hybridization results are highly reproducible.