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. Author manuscript; available in PMC: 2017 Jun 1.
Published in final edited form as: Stem Cells. 2016 Mar 15;34(6):1626–1636. doi: 10.1002/stem.2307

Figure 4. BVES regulates intestinal stem cell dynamics and Wnt signaling ex vivo.

Figure 4

qRT-PCR analysis revealed increases in expression of (A) CBC stem cell markers in Bves−/− enteroids compared to WT. Lgr5, Ascl2, Olfm4, Nanog, and Sox9 mRNA levels in Bves−/− enteroids compared to WT. qRT-PCR analysis also revealed increases in (B) +4 stem cell markers Bmi1 and Lrig1 mRNA levels in Bves−/− enteroids compared to WT with no significant differences in TERT expression (P=0.11). Expression of (C) Wnt ligands, (D) Wnt receptors, and (E) Wnt targets were significantly elevated in the Bves−/− enteroids. *P<0.05, **P<0.01 (n=6).