Fig. 1.

The effect of increasing concentrations of TBBPA (0.1, 1, 10, 50, and 100 μM) on the DCF fluorescence in cell-free PBS without Ca²+ and Mg²+ buffer and with addition of NAC after 30 min (A) and 60 min (B). Medium with 0.3 % hydrogen peroxide (H₂O₂) was used as a positive control. The data is expressed as the means ± SEM of four independent experiments, each of which consisted of eight replicates per treatment group. *p < 0.05, **p < 0.01, and ***p < 0.001 vs. the control. #p < 0.05, ##p < 0.01, and ###p < 0.001 vs. the group without NAC ROS scavenger