Figure 3.

Administration of ectosomes attenuates monosodium urate (MSU)-driven peritoneal inflammation. B6 mice were injected intraperitoneally with 3 mg MSU. Where indicated, mice were preinjected with 2×10⁷ BM-Ecto or PMN-Ecto intraperitoneally 2 h prior to MSU stimulation. Alternatively, mice were preinjected with 75 nM (approximately 2×10⁷) of phosphatidylserine (PS)-liposomes or phosphatidylcholine (PC)-liposomes. Control groups received BM-/PMN-Ecto or PS-/PC-liposome injections intraperitoneally followed by NaCl instead of MSU. (A and C) IL-1β in peritoneal lavage fluid was determined by ELISA 4 h after MSU stimulation. (B and D) The number of infiltrating PMN into the peritoneum 14 h after MSU stimulation was determined as indicated in figure 2D. n=6–8 per group pooled from at least three independent experiments, *p<0.05, **p<0.01, ***p<0.001. Mean±SEM is shown.