Figure 2.

TRAF6^IEC-KO mice develop chronic colon inflammation upon dextran sodium sulfate (DSS) treatment. (A) TRAF6^FL (n=8) and TRAF6^IEC-KO (n=9) mice were administered 2% DSS for 6 days followed by normal drinking water with endoscopies at the indicated time points until day 60 when they were sacrificed. (B) Body weight change and final survival at day 60. (C) Mean endoscopic index of colitis severity of TRAF6^FL and TRAF6^IEC-KO mice at indicated time points of the chronic DSS protocol. (D) Representative endoscopic pictures from TRAF6^FL and TRAF6^IEC-KO mice at day 54 of the chronic DSS colitis protocol. (E) Representative H&E stained colon cross-sections of TRAF6^FL and TRAF6^IEC-KO mice at day 60 of the chronic DSS colitis protocol. Bars, 100 μm. (F) Histological tissue damage and inflammation scoring at day 60 of the chronic DSS protocol. All data shown are representative of two independent experiments. All statistical analyses were performed with unpaired two-sided Student's t tests with unequal variance.