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. 2015 Mar 23;65(6):1015–1023. doi: 10.1136/gutjnl-2014-308003

Figure 4.

Figure 4

The fibroblast growth factor-2 (FGF-2) related mediator FGF-1 does not enhance HCV. (A) Huh-7.5 cells were electroporated with the Fluc-Jc1. Six hours later media supplemented with the indicated concentration of FGF-1 or FGF-2 was added. Cells were lysed and luciferase measured after 48 h of incubation. (B) Supernatants from the experiment represented in panel A were collected 48 h after electroporation, filtered and used to infect naïve Huh-7.5 cells. After 6 h media was exchanged and the cells were incubated without any mediators for 48 h before lysis and quantification of luciferase activity. In all panels dots represent the mean±SD of n=4 replicates from two independent experiments with the mean value obtained without any mediator set to 100%.