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. 2015 Mar 23;65(6):1015–1023. doi: 10.1136/gutjnl-2014-308003

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Fibroblast growth factor-2 (FGF-2) stimulation of HCV replication is dependent on expression of FGF receptor 3 (FGFR3). (A) Normally FGFR3-negative Huh6 cells were transduced with a lentiviral vector encoding FGFR3 or mock. FGFR3 expression was then assessed by immunoblotting. (B) Huh-6/mock and Huh6/FGFR3 cells were electroporated with the Fluc-Jc1. Five hours later media supplemented with the indicated concentration of FGF-2 was added. Cells were lysed and luciferase measured after 48 h of incubation. (C) Normally FGFR3-positive Huh-7.5 cells were transduced to express a short hairpin RNA (shRNA) against FGFR3 or an irrelevant control shRNA. FGFR3 expression was then assessed by immunoblotting. (D) The cells shown in panel C were infected with HCVcc and exposed to the indicated concentrations of FGF-2. Cells were lysed and luciferase measured after 48 h of incubation. In all panels bars represent the mean±SD of n=6 replicates from three independent experiments with the mean value obtained without FGF-2 on control cell lines set to 100%.