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letter
. 2016 Feb 24;71(6):565–567. doi: 10.1136/thoraxjnl-2015-208215

Figure 2.

Figure 2

Idiopathic pulmonary fibrosis (IPF) fibroblasts were pretreated with 0 μM or 50 μM caffeine for 30 min then stimulated with 0 ng/mL or 2 ng/mL TGFβ for 24 h and (A) PAI1; (B) ACTA2; (C) TGFB1 gene expression measured. Data are expressed as mean fold change over control (0 h, 0 ng/mL TGFβ)±SEM from experiments performed on cells from three individual donors. IPF fibroblasts were pretreated with 0 μM or 10 μM roflumilast for 30 min then stimulated with 0 ng/mL or 2 ng/mL TGFβ for 24 h and (D) PAI1; (E) ACTA2; (F) TGFB1 gene expression measured. Data are expressed as mean fold change over control (0 h, 0 ng/mL TGFβ)±SEM from experiments performed on cells from four individual donors. (G) Precision-cut lung slices (PCLS) were prepared from the lungs of saline-treated and bleomycin-treated mice and collagen levels measured after 5 days in ex vivo culture. Data are expressed as mean collagen (mg) per mg of lung tissue±SEM from n=16 PCLS/group. (H) PCLS were prepared from the lungs of bleomycin-treated or saline-treated mice and treated for 5 days ex vivo with 0 μM, 25 μM, 50 μM and 100 μM caffeine. Data are expressed as mean collagen (mg) per mg of lung tissue±SEM from n=6 bleomycin PCLS or n=2 saline PCLS. *p<0.05, **p<0.01, ****p<0.0001.