Figure 2.

E2F Transcription and Active Protein Synthesis Are Required to Maintain and/or Upregulate the Levels of Checkpoint Proteins

Asterisks mark unspecific bands.

(A) Western blot of WCE (whole-cell extract), RPE1 cells, treatment, and times shown.

(B) Western blot of WCE, HEK293 cells, treatment, and times shown.

(C) Quantification of (A) (RPE1) and (B) (HEK293), normalized to GAPDH and 0 hr.

(D) Western blot of WCE, RPE1 TetON E2F6 cells, treatment, and times shown. E2F6 overexpression, Doxy.

(E) Western blot of WCE, HEK293 T-Rex E2F6 cells, treatment, and times shown.

(F) Western blot of WCE, HEK293 T-Rex E2F6 cells, treatment, and times shown.

See also Figure S3.