Figure 4.

E2F Activity Is Sufficient to Prevent DNA Damage and Rescue Checkpoint Functions in Cells with a Compromised Checkpoint Response

(A) Western blot of WCE, RPE1 cells transfected with siRNA shown −/+ 4 hr HU,.

(B) Column scatter graph of mean intensity of γH2AX of individual nuclei, RPE1 cells from 4A. Transfected with siRNA are shown −/+ 4 hr HU. Mean is in red. ^∗∗∗∗p < 0.0001; ^∗∗∗p < 0.001; ^∗p < 0.05 with Wilcoxon. Arrows show change of mean.

(C) Representative images for (B). The scale bar represents 20 μm and is the same for all.

(D) Western blot of chromatin preparation, RPE1 cells transfected with siRNA shown, HU 4 hr.

(E) Schematic and representative images for (F), T98G cells. The scale bar represents 10 μm.

(F) Bar graphs of length of DNA tracks before and after HU treatment with siRNA and treatments shown. Table is of differences in mean length (after-before). p, difference with Wilcoxon.

(G) RS checkpoint activation sustains E2F transcription. This transcriptional response is essential and sufficient during RS to maintain optimal protein levels and important functions of the checkpoint response to prevent RS-induced DNA damage.

See also Figures S4 and S5.