Figure 4.

An siRNA Screen for Genes Affecting Transcription upon UV Irradiation

(A) The experimental approach.

(B) Typical examples of siRNAs that result in either (left) “low transcription,” or (right) “high transcription,” relative to the controls (middle). Different putative causes (not necessarily mutually exclusive) of the outcome are listed below arrows.

(C) Nascent transcription profiles across a cell population in the absence of UV irradiation, and in the examples from (B), used to identify siRNAs giving rise to low and high transcription, respectively. EU intensity (y axis) across the population of cells in an individual plate well (x axis) is shown.

(D) Graphical representation of the screen result. High transcribers are labeled green, and low transcribers are red. Specific genes are indicated. Other proteins can be searched at http://www.biologic-db.org.