Figure 7.

Involvement of STK19 in the Transcription-Related DNA Damage Response

(A and B) Lack of normal transcription recovery in cells lacking STK19.

(C–E) Cells lacking STK19 are UV-sensitive. The individual siRNAs that knock down STK19 (D) also give rise to UV sensitivity (E). The result of CSB knockdown is shown for comparison. NT, non-targeting siRNA.

(F) Recruitment of GFP-tagged STK19 to DNA damage induced by laser micro-irradiation in a diffraction-limited spot (blue arrows) or stripe (yellow arrows). Cells were imaged immediately before and 2 hr after micro-irradiation.

See also Figures S4 and S5 and the Supplemental Experimental Procedures for details.