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. 2016 Jun 1;90(5):1000–1015. doi: 10.1016/j.neuron.2016.04.046

Figure 4.

Figure 4

DIC1a Interacts with the Central Stalk Region of KIF5 Heavy Chains

(A) Western blot (WB) of COS cells cotransfected with mCherry-tagged DIC1a and myc-tagged KIF5A-C constructs as indicated followed by immunoprecipitation with anti-myc.

(B) Schematic of KIF5 showing the constructs used in (C), the sequence isolated by yeast two-hybrid screen and the resulting consensus region for binding DIC relative to key domains: motor domain, coiled-coil, cargo binding domain (CBD), central hinge, and KLC binding region.

(C) Western blot (WB) of COS cells cotransfected with mCherry-tagged DIC1a and GFP-tagged KIF5C “head,” “stalk,” and “tail” constructs shown in (B) followed by immunoprecipitation with anti-GFP.