DIC1a Interacts with the Central Stalk Region of KIF5 Heavy Chains
(A) Western blot (WB) of COS cells cotransfected with mCherry-tagged DIC1a and myc-tagged KIF5A-C constructs as indicated followed by immunoprecipitation with anti-myc.
(B) Schematic of KIF5 showing the constructs used in (C), the sequence isolated by yeast two-hybrid screen and the resulting consensus region for binding DIC relative to key domains: motor domain, coiled-coil, cargo binding domain (CBD), central hinge, and KLC binding region.
(C) Western blot (WB) of COS cells cotransfected with mCherry-tagged DIC1a and GFP-tagged KIF5C “head,” “stalk,” and “tail” constructs shown in (B) followed by immunoprecipitation with anti-GFP.