Figure 6.

Discrete Populations of Dynein-GFP Move with an Anterograde Bias in the Axon

(A) Example axon before (t = –1) and after (t = 0) bleaching. The relative position of the soma is indicated.

(B) Midpoint calculation of dynein-GFP photo-protected region for t = 0. The relative intensity of the axon line scan at t = 0, showing raw data (gray dots) and rolling average (green line). Anterograde and retrograde fronts of the photo-protected population are defined as the positions along the axon where the intensity is half-maximal. The midpoint is defined as halfway between these two intercepts.

(C) Line scans and midpoints for all time points of the movie, demonstrating a displacement toward the distal axon with time. Color changes with time indicated by the scale bar, top left.

(D) Kymograph of dynein-GFP axon from (A) showing anterograde movement of the photo-protected region, over plotted with the calculated midpoints and intercepts from (C).

(E) The mean displacement of the midpoint through time (green line) from n = 11 neurons, ±SEM (green ribbon).